PDF DatasheetHuman, Rat, and Broad Species Reactivity Anti-Cytokeratin Antibody Product Attributes
Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: KRTL/1077 + KRTH/1076
Clone KRTL/1077 + KRTH/1076 Host and Isotype: Mouse IgG
Anti-Human, Rat, and Broad Species Reactivity Cytokeratin Positive Control Sample: Skin, Adeno- or Squamous carcinomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human KRT77, KRT76 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin Previously Observed Antibody Staining Patterns
| Cytokeratin General Information | |
|---|---|
| Molecular Weight | |
| 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) | |
| Curated Database and Bioinformatic Data | |
| Entrez Gene ID | 374454, 51350 |
| Ensemble Gene ID | ENSG00000189182, ENSG00000185069 |
| RefSeq Protein Accession(s) | NP_778253, XP_011536591, XP_011536590, NP_056932 |
| RefSeq mRNA Accession(s) | XM_011538288, NM_175078NM_015848, |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_012322, NG_012420, NC_000012, NC_018923 |
| UniProt ID(s) | Q7Z794, Q0IIN1, Q01546 |
| UniGene ID(s) | Q7Z794, Q0IIN1, Q01546 |
| HGNC ID(s) | 20411, 24430 |
| Cosmic ID(s) | KRT77, KRT76 |
| KEGG Gene ID(s) | hsa:374454, hsa:51350 |
| PharmGKB ID(s) | PA134896049, PA147357785 |
| General Description of Cytokeratin. | |
| Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW), basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16,, CK19. Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer,, esophageal cancer. It has been used to characterize the source of various neoplasms, to study the distribution of cytokeratin containing cells in epithelia during normal development, during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal, abnormal human tissues, has shown high sensitivity in the recognition of epithelial cells, carcinomas. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin, pan Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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