PDF DatasheetHuman, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken Anti-Cytokeratin Antibody Product Attributes
Species: Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml for 2 hours at RT), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: SPM115 + SPM116
Clone SPM115 + SPM116 Host and Isotype: Mouse IgG
Anti-Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken Cytokeratin Positive Control Sample: Skin, Adeno- or Squamous carcinomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human epidermal keratin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin Previously Observed Antibody Staining Patterns
| Cytokeratin General Information | |
|---|---|
| Alternate Names | |
| K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39, anti-K1B antibody, anti-KRT1B antibody, anti-Keratin antibody, anti-type II cytoskeletal 1b antibody, anti-K77 antibody, anti-CK-1B antibody, anti-Keratin 1B antibody, anti-Keratin-77 antibody, anti-Cytokeratin-1B antibody, anti-Type-II Keratin Kb39 antibody | |
| Molecular Weight | |
| 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) | |
| Chromosomal Location | |
| 17q21.2 (KRT77) & 12q13.13 (KRT76) | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT77 & KRT76 |
| Entrez Gene ID | 374454, 51350 |
| Ensemble Gene ID | ENSG00000189182, ENSG00000185069 |
| RefSeq Protein Accession(s) | NP_778253, XP_011536591, XP_011536590, NP_056932 |
| RefSeq mRNA Accession(s) | XM_011538288, NM_175078NM_015848, |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_012322, NG_012420, NC_000012, NC_018923 |
| UniProt ID(s) | Q7Z794, Q0IIN1, Q01546 |
| UniGene ID(s) | Q7Z794, Q0IIN1, Q01546 |
| HGNC ID(s) | 20411, 24430 |
| Cosmic ID(s) | KRT77, KRT76 |
| KEGG Gene ID(s) | hsa:374454, hsa:51350 |
| PharmGKB ID(s) | PA134896049, PA147357785 |
| General Description of Cytokeratin. | |
| Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW), basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer,, esophageal cancer. It has been used to characterize the source of various neoplasms, to study the distribution of cytokeratin containing cells in epithelia during normal development, during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal, abnormal human tissues, has shown high sensitivity in the recognition of epithelial cells, carcinomas. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin, pan Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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