PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Fascin-1 Information for Pathologists
Summary:
Fascin-1 is an actin bundling protein with important role in cell motility and adhesion. High expression in tumors is generally a poor prognostic marker. Fascin-1 is present in specialized cells with extensive surfaces or migratory potential, such as neurons, glia, dendritic cells, macrophages, skeletal and smooth muscle, endothelial cells but is generally absent in normal epithelial cells.
Common Uses By Pathologists:
Facin-1 staining is generally observed in carcinoma of biliary tract, breast, colon, lung, ovary, pancreas, and skin. Follicular dendritic cell tumors, Hodgkin lymphoma-classic subtype (highly sensitive), interdigitating dendritic cell tumors, Langerhan cell histiocytosis, and urothelial carcinoma (noninvasive papillary or invasive) also express Fascin-1.
| Fascin-1 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 54.5 kDa | |
| Chromosomal Location | |
| p22.1 [chr: 7] [chr_start: 5592816] [chr_end: 5606655] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FSCN1 |
| Entrez Gene ID | 6624 |
| RefSeq Protein Accession(s) | NP_003079 |
| RefSeq mRNA Accession(s) | ; NM_003088 |
| RefSeq Genomic Accession(s) | NC_000007; NG_030004 |
| UniProt ID(s) | Q16658 |
| PharmGKB ID(s) | PA128394534 |
| KEGG Gene ID(s) | hsa:6624 |
| General Description of Fascin-1 . | |
| Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases. | |
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