PDF DatasheetHuman, Monkey, and Mouse Anti-FOXP3 / Scurfin Antibody Product Attributes
Species: Human, Monkey, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-FOXP3 / Scurfin Antibody Clone: FXP3/197
Clone FXP3/197 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, and Mouse FOXP3 / Scurfin Positive Control Sample: Tonsil or Testis
Cellular Localization of Antibody Nuclear, Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length FOXP3 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
FOXP3 / Scurfin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-FOXP3 antibody staining is expected to be primarily localized to the nucleoplasm.
Observed Antibody Staining Data By Tissue Type:
Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| FOXP3 / Scurfin General Information | |
|---|---|
| Alternate Names | |
| FOXP3, forkhead box P3, scurfin | |
| Molecular Weight | |
| 47-55kDa | |
| Chromosomal Location | |
| Xp11.23 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FOXP3 |
| Entrez Gene ID | 50943 |
| Ensemble Gene ID | ENSG00000049768 |
| RefSeq Protein Accession(s) | XP_016885054, XP_016885056, XP_006724596, XP_016885055, NP_001107849, XP_011542218, NP_054728 |
| RefSeq mRNA Accession(s) | XM_011543916, XM_017029566, XM_006724533, XM_017029567, NM_001114377, NM_014009 |
| RefSeq Genomic Accession(s) | NG_007392, NC_018934, NC_000023 |
| UniProt ID(s) | Q9BZS1, B7ZLG1 |
| UniGene ID(s) | Q9BZS1, B7ZLG1 |
| HGNC ID(s) | 6106 |
| Cosmic ID(s) | FOXP3 |
| KEGG Gene ID(s) | hsa:50943 |
| PharmGKB ID(s) | PA201094 |
| General Description of FOXP3 / Scurfin. | |
| Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1, FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal, cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. | |
Limitations and Warranty
enQuire Bio’s FOXP3 / Scurfin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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