.jpg "Kappa Light Chain IHC human Tonsil (Kap-56)")
PDF DatasheetHuman Anti-Kappa Light Chain Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-2.0ug of antibody/million cells in 0.1ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Kappa Light Chain Antibody Clone: Kap-56
Clone Kap-56 Host and Isotype: Mouse IgG1 kappa
Anti-Human Kappa Light Chain Positive Control Sample: 293T, Raji or hPBL cells. Tonsil or Spleen
Cellular Localization of Antibody Cell Surface, cytoplasmic, secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human lymphocytes stimulated
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Kappa Light Chain Previously Observed Antibody Staining Patterns
| Kappa Light Chain General Information | |
|---|---|
| Alternate Names | |
| Immunoglobulin kappa constant, IGKC | |
| Molecular Weight | |
| ~22.5kDa | |
| Chromosomal Location | |
| 2p11.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGKC |
| Entrez Gene ID | 3514 |
| RefSeq Genomic Accession(s) | NC_000002, NG_000834, NC_018913 |
| UniGene ID(s) | |
| HGNC ID(s) | 5716 |
| Cosmic ID(s) | IGKC |
| General Description of Kappa Light Chain. | |
| This MAb is specific to kappa light chain of immunoglobulin, shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas,, certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. | |
Limitations and Warranty
enQuire Bio’s Kappa Light Chain Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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