.jpg "MAGE-1 IHC human Testicular Carcinoma (MZ2E 838)")
PDF DatasheetHuman Anti-MAGE-1 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MAGE-1 Antibody Clone: MZ2E/838
Clone MZ2E/838 Host and Isotype: Mouse IgG1 kappa
Anti-Human MAGE-1 Positive Control Sample: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian,, renal cell carcinomas.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MAGEA1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
MAGE-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MAGEA1 antibody staining is expected to be primarily localized to the cytosol.
Observed Antibody Staining Data By Tissue Type:
Variations in MAGE-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis. Low, but measureable presence of MAGE-1 could be seen in. We were unable to detect MAGE-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| MAGE-1 General Information | |
|---|---|
| Alternate Names | |
| Melanoma-associated antigen 1, MAGEA1 | |
| Molecular Weight | |
| 42-46kDa | |
| Chromosomal Location | |
| Xq28 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAGEA1 |
| Entrez Gene ID | 4100 |
| Ensemble Gene ID | ENSG00000198681 |
| RefSeq Protein Accession(s) | NP_004979 |
| RefSeq mRNA Accession(s) | NM_004988, |
| RefSeq Genomic Accession(s) | NG_013243, NC_018934, NC_000023 |
| UniProt ID(s) | P43355 |
| UniGene ID(s) | P43355 |
| HGNC ID(s) | 6796 |
| Cosmic ID(s) | MAGEA1 |
| KEGG Gene ID(s) | hsa:4100 |
| PharmGKB ID(s) | PA30542 |
| General Description of MAGE-1. | |
| Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with other members of MAGE-family. Human malignant neoplasms carry rejection antigens that are recognized by the patients’ autologous, tumor directed, specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas, primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas), not on normal tissues, the encoded antigen may serve as a marker of early detection, target for cancer immunotherapy. | |
Limitations and Warranty
enQuire Bio’s MAGE-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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