PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:20-1:40 |
| Pretreatment | No Pretreatment Required |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Myelin Basic Protein Information for Pathologists
Summary:
MBP is important in myelination of nerves (reported in both central and peripheral nerves). Myelination of nerves and the formation of the myelin sheath is critical to rapid conduction of neural impulses along axons. Several studies have suggested that auto antibodies against MBP are linked to development of multiple sclerosis (MS).
Notable Clinical Features:
Myelin Basic protein in CSF above levels of 4 ng/ml is indicative myelin breakdown which is linked to MS. MBP staining is reported as a sensitive marker of myelenation.
Common Uses By Pathologists:
Neuromas, Neurofi bromas, and Neurogenic Sarcomas have been reported as staining postive for MBP. However, other spindle-cell neoplasms are typically negative. MBP staining of Granular-cell tumors is useful in supportinga Schwann-cell derivation of these lesions. Unlike other nervous system proteins such as GFAP and S-100, MBP has not been demonstrated in melanocytes or tumors derived from melanocytes.
| Myelin Basic Protein General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 33.1 kDa | |
| Chromosomal Location | |
| q23 [chr: 18] [chr_start: 76978827] [chr_end: 77133683] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MBP |
| Entrez Gene ID | 4155 |
| RefSeq Protein Accession(s) | NP_001020263; NP_002376; NP_001020252; NP_001020271; XP_016881269; NP_001020261; NP_001020272 |
| RefSeq mRNA Accession(s) | NM_001025081; XM_024451185; XM_024451186; XM_024451188; XR_001753201; NM_002385; NM_001025090; NM_001025094; XM_024451187; XR_001753202; XM_017025778; NM_001025092; NM_001025101; XM_017025780; XM_024451189; NM_001025100; NM_001025098 |
| RefSeq Genomic Accession(s) | ; NC_000018 |
| UniProt ID(s) | P02686 |
| PharmGKB ID(s) | PA30667 |
| KEGG Gene ID(s) | hsa:4155 |
| Associated Diseases (KEGG IDs) | The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). |
| General Description of Myelin Basic Protein . | |
| This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody stains peripheral nerves also. | |
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