PDF Datasheetother Myelin Basic Protein Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Myelin Basic Protein based upon sequence from other
Host: QP12658 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Myelin Basic Protein was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP12658.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGNHAGK RELNAEKAST NSETNRGESE KKRNLGELSR TTSEDNEVFG EADANQNNGT SSQDTAVTDS KRTADPKNAW QDAHPADPGS RPHLIRLFSR DAPGREDNTF KDRPSESDEL QTIQEDSAAT SESLDVMASQ KRPSQRHGSK YLATASTMDH ARHGFLPRHR DTGILDSIGR FFGGDRGAPK RGSGKVSSEE
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The MBP solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant other Myelin Basic Protein General Information | |
|---|---|
| Alternate Names | |
| Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein. | |
| Molecular Weight | |
| 23.9 kDa | |
| Chromosomal Location | |
| q13.2 on chromosome 22 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TEF |
| Entrez Gene ID | 7008 |
| UniProt ID(s) | Q10587 |
| COSMIC ID Link(s) | TEF |
| KEGG Gene ID(s) | hsa:7008 |
| General Description of Recombinant other Myelin Basic Protein. | |
| MBP Recombinant produced in E. coli is a single polypeptide chain containing 220 amino acids (1-197) and having a molecular mass of 23.9kDa. MBP is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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