PDF DatasheetHuman, Mouse, Rat, and Chicken Anti-MyoD1 Antibody Product Attributes
Species: Human, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MyoD1 Antibody Clone: SPM427
Clone SPM427 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, and Chicken MyoD1 Positive Control Sample: Rhabdomyosarcoma
Cellular Localization of Antibody Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant mouse MyoD1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
MyoD1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MYOD1 antibody staining is expected to be primarily localized to the nuclear bodies and nucleoplasm.
| MyoD1 General Information | |
|---|---|
| Alternate Names | |
| MyoD, MyoD1 | |
| Molecular Weight | |
| 45kDa | |
| Chromosomal Location | |
| 11p15.1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MYOD1 |
| Entrez Gene ID | 4654 |
| Ensemble Gene ID | ENSG00000129152 |
| RefSeq Protein Accession(s) | NP_002469 |
| RefSeq mRNA Accession(s) | NM_002478 |
| RefSeq Genomic Accession(s) | NC_018922, NC_000011 |
| UniProt ID(s) | P15172 |
| UniGene ID(s) | P15172 |
| HGNC ID(s) | 7611 |
| Cosmic ID(s) | MYOD1 |
| KEGG Gene ID(s) | hsa:4654 |
| PharmGKB ID(s) | PA31416 |
| General Description of MyoD1. | |
| Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma, a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium, also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas, alveolar soft part sarcomas. | |
Limitations and Warranty
enQuire Bio’s MyoD1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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