PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
4-Oct Information for Pathologists
Summary:
OCT-4 (also Octamer-binding transcription factor 4) is an 18 kDa transription factor encoded by POU5F1. It is considered the master gene in pluripotency and embryonic stem cell regulation. It regulates self-renewal and differentiation of embryonic stem cells, include germ cells.
Common Uses By Pathologists:
OCT-4 is a Nuclear stain. It is highly sensitive and specific for primary intracranial germinomas which may be benign or malignant. Positive staining for OCT-4 is generally related to poor prognostis in esophageal squamous cell carcinoma and possibly in non-small cell lung cancer (NSCLC).
| OCT-4 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 38.6 kDa | |
| Chromosomal Location | |
| [chr: CHR_HSCHR6_MHC_QBL_CTG1] [chr_start: 31156777] [chr_end: 31163150] [strand: -1]; p21.33 [chr: 6] [chr_start: 31164337] [chr_end: 31180731] [strand: -1]; [chr: CHR_HSCHR6_MHC_DBB_CTG1] [chr_start: 31158060] [chr_end: 31164434] [strand: -1]; [chr: CHR_HSCHR6_MHC_COX_CTG1] [chr_start: 31156880] [chr_end: 31163254] [strand: -1]; [chr: CHR_HSCHR6_MHC_MANN_CTG1] [chr_start: 31209245] [chr_end: 31215620] [strand: -1]; [chr: CHR_HSCHR6_MHC_MCF_CTG1] [chr_start: 31242859] [chr_end: 31249234] [strand: -1]; [chr: CHR_HSCHR6_MHC_SSTO_CTG1] [chr_start: 31159218] [chr_end: 31165590] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | POU5F1 |
| Entrez Gene ID | 5460 |
| RefSeq Protein Accession(s) | NP_002692; NP_001272916 |
| RefSeq mRNA Accession(s) | NM_001173531; NM_001285987; NM_001285986; NM_203289; NM_002701 |
| RefSeq Genomic Accession(s) | NT_167248; NT_167249; NT_113891; NT_167245; NT_167246; NC_000006; NT_167247 |
| UniProt ID(s) | Q01860 |
| PharmGKB ID(s) | PA33545 |
| KEGG Gene ID(s) | hsa:5460 |
| General Description of OCT-4 . | |
| OCT-4 (octamer-binding transcription factor 4) also known as POU5F1 is a homeodomain transcription factor. It is involved in self-renewal of undifferentiated embryonic stem cells. It is frequently used as a marker for undifferentiated cells. OCT-4 expression must be closely regulated; too much or too little will cause differentiation of the cells. OCT-4 is one of the transcription factors used to create induced pluripotent stem cells. OCT-4 is sensitive and specific marker for germ cell tumors like seminomas, gonadoblastoma, germinoma and embryonal carcinomas but not for differentiated cells of nonseminomas, i.e. teratomas, yolk sac tumors and choriocarcinomas. OCT-4 can detect both primary and metastatic germ cell tumors. | |
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