PDF DatasheetAntibody DISCONTINUED
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:100-1:200 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
p27Kip1 Information for Pathologists
Summary:
Cyclin dependent kinase inhibitor leading to cell cycle arrest in G1 phase. Member of Cip/Kip family of proteins. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. Interpretation Nuclear stain.
| p27Kip1 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 22.1 kDa | |
| Chromosomal Location | |
| p13.1 [chr: 12] [chr_start: 12715058] [chr_end: 12722369] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDKN1B |
| Entrez Gene ID | 1027 |
| RefSeq Protein Accession(s) | NP_004055 |
| RefSeq mRNA Accession(s) | ; NM_004064 |
| RefSeq Genomic Accession(s) | NG_016341; NC_000012 |
| UniProt ID(s) | P46527 |
| PharmGKB ID(s) | PA105 |
| KEGG Gene ID(s) | hsa:1027 |
| Associated Diseases (KEGG IDs) | Multiple endocrine neoplasia 4 (MEN4) [MIM:610755]: Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. {ECO:0000269|PubMed:17030811}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of p27Kip1 . | |
| p27Kip1 is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27Kip1 binds to and inhibits CyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 complexes. P27Kip1 is regulated by phosphorylation and the site of phosphorylation determines the fate of the protein, whether it is degraded or exported out of the nucleus to the cytoplasm. The expression of p27Kip1 is high in normal cells. Down regulation of p27Kip1 is found in many types of cancer, and decreased expression of p27Kip1 is associated with poor prognosis in several tumors, including lung, breast, colorectal and prostate. | |
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