PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Prostate Specific Antigen Information for Pathologists
Summary:
Kallikrein related serine protease produced by secretory epithelium, drains into ductal system. Cleaves and liquefies seminal coagulum formed after ejaculation. Clinical features Serum PSA > 4 seen in 80% with histologically documented cancer but also in 25 – 30% with nodular hyperplasia, prostatitis, infarcts, prostatic massage, cystoscopy. Elevated in 2 of 18 post-race marathon runners (Arch Pathol Lab Med 2003;127:345).
Common Uses By Pathologists:
Glycoprotein detected in benign and malignant prostate tissue. Identifies prostatic origin of most metastatic tumors, differentiates between prostatic and urothelial carcinomas. Non-prostate tumors are usually weak / negative for PSA / PAP. PSA is more sensitive (intense, diffuse staining) and specific than PAP. Microscopic (histologic) images
| Prostate Specific Antigen General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 28.7 kDa | |
| Chromosomal Location | |
| q13.33 [chr: 19] [chr_start: 50854915] [chr_end: 50860764] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KLK3 |
| Entrez Gene ID | 354 |
| RefSeq Protein Accession(s) | NP_001025219; NP_001025218; NP_001639 |
| RefSeq mRNA Accession(s) | NM_001648; NM_001030047; NM_001030050; NM_001030048; NM_001030049 |
| RefSeq Genomic Accession(s) | NG_011653; NC_000019; |
| UniProt ID(s) | P07288 |
| PharmGKB ID(s) | PA164741810 |
| KEGG Gene ID(s) | hsa:354 |
| General Description of Prostate Specific Antigen . | |
| Prostate-specific antigen (PSA) is a serine protease member of the human glandular kallikrein family. It is synthesized in the prostate ductal and acinar epithelium and diffused into serum. It is found in normal, hyperplastic, and malignant prostate tissue. Low expression of PSA has been reported in other normal or tumor tissues such as urethral, periurethral,and perianal glands, salivary duct carcinoma, and rare mammary carcinomas. Although low PSA expression has been found in other tissues, PSA is still a specific and sensitive marker for immunohistochemical analysis of tumors with prostate epithelial cell differentiation. It is valuable in the identification of metastatic tumors of prostatic origin. | |
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