PDF DatasheetHuman, Rat, and Mouse Anti-Spectrin beta III Antibody Product Attributes
Spectrin beta III Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-SPTBN2 antibody staining is expected to be primarily localized to the cell junctions and cytosol.
Observed Antibody Staining Data By Tissue Type:
Variations in Spectrin beta III antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, Purkinje cells in the cerebellum, neuropil in cerebral cortex, glandular cells in the cervix, uterine, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the prostate, keratinocytes in skin and epidermal cells in the skin. Low, but measureable presence of Spectrin beta III could be seen inglandular cells in the breast, respiratory epithelial cells in the bronchus, glandular cells in the endometrium, epididymis and gallbladder, hepatocytes in liver, macrophages in lung, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, Langerhans in skin, cells in the red pulp in spleen and cells in the seminiferous ducts in testis. We were unable to detect Spectrin beta III in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Spectrin beta III expression as measured by anti-Spectrin beta III antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | + | ++ | ++ | ++ | ++ | + | + | + | + | – | – | ++ | + | +++ | – | ++ | + | – | +++ | + |
| SPTBN2 Variability | +++ | ++ | ++ | + | ++ | +++ | ++ | +++ | ++ | + | ++ | +++ | ++ | + | ++ | ++ | ++ | ++ | ++ | +++ |
| Spectrin beta III General Information | |
|---|---|
| Alternate Names | |
| Spectrin beta chain, brain 2, SPTBN2 | |
| Molecular Weight | |
| 246kDa | |
| Chromosomal Location | |
| 11q13.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SPTBN2 |
| Entrez Gene ID | 6712 |
| Ensemble Gene ID | ENSG00000173898 |
| RefSeq Protein Accession(s) | XP_011543518, XP_016873665, XP_016873666, XP_005274249, XP_006718734, XP_005274250, XP_006718732, XP_016873663, NP_008877, XP_011543519, XP_016873667, XP_016873664 |
| RefSeq mRNA Accession(s) | XM_011545217, XM_017018178, XM_011545216, XM_017018177, XM_005274192, XM_006718669, XM_017018176, XM_017018174, XM_017018175, XM_005274193, NM_006946 |
| RefSeq Genomic Accession(s) | NG_016150, NC_000011, NC_018922 |
| UniProt ID(s) | O15020 |
| UniGene ID(s) | O15020 |
| HGNC ID(s) | 11276 |
| Cosmic ID(s) | SPTBN2 |
| KEGG Gene ID(s) | hsa:6712 |
| PharmGKB ID(s) | PA36105 |
| General Description of Spectrin beta III. | |
| Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin ? and spectrin ? are present in erythrocytes, whereas spectrin ? II (also designated fodrin ?) and spectrin ? I (also designated fodrin ?) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ? III is highly homologous to both spectrin ? I and spectrin ? II. Spectrin ? III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. | |
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