Anti-SUMO-1 Antibody Clone SPM571

$ 429.00

SKU: 7341-MSM1X
Species: Human and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Human and Broad Species Reactivity Anti-SUMO-1 Antibody Product Attributes

Species: Human and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-SUMO-1 Antibody Clone: SPM571
Clone SPM571 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Broad Species Reactivity SUMO-1 Positive Control Sample: Breast carcinoma
Cellular Localization of Antibody Predominantly nuclear with some cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human SUMO1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

SUMO-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-SUMO1 antibody staining is expected to be primarily localized to the nuclear bodies, nuclear membrane, nucleoli and nucleus.

Observed Antibody Staining Data By Tissue Type:

Variations in SUMO-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland, hematopoietic cells in the bone marrow, adipocytes in breast, respiratory epithelial cells in the bronchus, glandular cells in the duodenum, endometrium, epididymis, fallopian tube and gallbladder, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the rectum, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, glandular cells in the small intestine, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, cells in the seminiferous ducts in testis, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the adrenal gland, hematopoietic cells in the bone marrow, adipocytes in breast, respiratory epithelial cells in the bronchus, glandular cells in the duodenum, endometrium, epididymis, fallopian tube and gallbladder, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the rectum, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, glandular cells in the small intestine, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, cells in the seminiferous ducts in testis, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of SUMO-1 could be seen inglandular cells in the appendix, lymphoid tissue in appendix, glial cells in the caudate nucleus and cerebral cortex, bile duct cells in the liver and Leydig cells in the testis. We were unable to detect SUMO-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of SUMO-1 expression as measured by anti-SUMO-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + ++ ++ + ++ ++ ++ + ++ ++ +++ ++ ++ + ++ ++ +++ ++ ++
SUMO1 Variability ++ ++ ++ ++ +++ ++ ++ +++ ++ ++ +++ ++ ++ ++ +++ ++ ++ ++ ++ +++

Limitations and Warranty

enQuire Bio’s SUMO-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-SUMO-1 Antibody Clone SPM571

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