PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Synaptophysin Antibody Clone: SP11
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody SP11 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide of human synaptophysin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Synaptophysin Information for Pathologists
Common Uses By Pathologists:
Common neuroendocrine marker (also chromogranin and CD56). Microscopic (histologic) images Images hosted on PathOut server:. Missing Image. Bladder: paraganglioma.
| Synaptophysin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 33.8 kDa | |
| Chromosomal Location | |
| p11.23 [chr: X] [chr_start: 49187804] [chr_end: 49200259] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | SYP |
| Entrez Gene ID | 6855 |
| RefSeq Protein Accession(s) | NP_003170 |
| RefSeq mRNA Accession(s) | ; NM_003179 |
| RefSeq Genomic Accession(s) | NC_000023; NG_012532 |
| UniProt ID(s) | P08247 |
| PharmGKB ID(s) | PA36288 |
| KEGG Gene ID(s) | hsa:6855 |
| Associated Diseases (KEGG IDs) | Mental retardation, X-linked 96 (MRX96) [MIM:300802]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. {ECO:0000269|PubMed:19377476}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Synaptophysin . | |
| This antibody recognizes a protein of 38kDa, identified as synaptophysin. It labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and gastric parietal cells. Neurons in the brain, spinal cord, and retina are also labeled. In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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