Antibody (Suitable for clinical applications)
Specification | Recommendation |
---|---|
Recommended Dilution (Conc) | 1:50-1:100 |
Pretreatment | Citrate Buffer pH 6.0 |
Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
TAG-72/CA 72-4 Information for Pathologists
Summary:
Definition: monoclonal antibody that recognizes tumor-associated glyocoprotein 72 (TAG-72), a mucin-like sugar and protein complex on the surface of many cancer cells. Antibody is directed against the Sialyl-Tn blood group antigen. Interpretation Cytoplasmic staining. Uses by pathologists
Common Uses By Pathologists:
Distinguish pulmonary adenocarcinoma (positive) from mesothelioma (negative) as part of a panel (Diagn Cytopathol 1997;17:115). Distinguish carcinoma in cytology specimens (positive) from benign effusions (usually negative, but may have focal staining, J Clin Pathol 1990;43:1026, Acta Cytol 1987;31:537, Diagn Cytopathol 2007;35:300). Confirm diagnosis of breast apocrine carcinoma (APMIS 2006;114:712).
TAG-72/CA 72-4 General Information | |
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Alternate Names | |
Molecular Weight | |
43.3 kDa | |
Chromosomal Location | |
[chr: I] [chr_start: 10159982] [chr_end: 10161883] [strand: -1] | |
Curated Database and Bioinformatic Data | |
Gene Symbol | tag-72 |
Entrez Gene ID | 182875 |
RefSeq Protein Accession(s) | NP_492674 |
RefSeq mRNA Accession(s) | NM_060273 |
RefSeq Genomic Accession(s) | NC_003279 |
UniProt ID(s) | Q9XVS1 |
KEGG Gene ID(s) | cel:CELE_C25A1.3 |
General Description of TAG-72/CA 72-4 . | |
This antibody is specific to an oncofetal antigen of >1,000 kDa known as tumor associated glycoprotein (TAG-72) or CA 72-4. This antibody defines the mucin carried sialylated-Tn antigen. This antibody stains the majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary and non-small cell lung cancer. |
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