PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
TdT Information for Pathologists
Summary:
Terminal deoxynucleotidyl transferase; also called terminal transferase. Nuclear DNA polymerase in thymic and small number of bone marrow cortical lymphocytes. Catalyzes addition of deoxynucleotides to 3′ OH ends of oligonucleotides or polydeoxynucleotides. Performs DNA synthesis using only single-stranded DNA as the nucleic acid substrate (Biochim Biophys Acta 2010;1804:1151). Used during immunoglobulin and T cell receptor rearrangement, adds diversity to antibody recognition sites.
Common Uses By Pathologists:
Interpretation: nuclear stain. Marker of B or T cell acute lymphoblastic leukemia/lymphoma (only rarely negative, Arch Pathol Lab Med 2000;124:92). Microscopic (histologic) images Image hosted on PathOut server:.
| TdT General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 58.5 kDa | |
| Chromosomal Location | |
| q24.1 [chr: 10] [chr_start: 96304409] [chr_end: 96338564] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DNTT |
| Entrez Gene ID | 1791 |
| RefSeq Protein Accession(s) | NP_004079; NP_001017520 |
| RefSeq mRNA Accession(s) | NM_001017520; NM_004088 |
| RefSeq Genomic Accession(s) | NC_000010 |
| UniProt ID(s) | P04053 |
| PharmGKB ID(s) | PA27449 |
| KEGG Gene ID(s) | hsa:1791 |
| General Description of TdT . | |
| Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that contributes to the generation of junctional diversity in antigen receptors of immature lymphocytes. TdT is expressed in lymphoid precursors of B- and T-cell lineage in thymus and bone marrow. Foci of TdT positive cells may be observed in peripheral lymphoid tissues. TdT is also present in malignant tumors of lymphoblastic lineage and thymoma. It is sensitive and specific marker for lymphoblastic lymphoma/leukemia. | |
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