Anti-TGF-alpha Antibody Clone TG86 + P/T1

$ 488.00

SKU: 7039-MSM5
Species: Human, Rabbit, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM
Mass Spec Validated?: Not MS Validated

 PDF Datasheet

Human, Rabbit, and Zebrafish Anti-TGF-alpha Antibody Product Attributes

Species: Human, Rabbit, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (2-4ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-TGF-alpha Antibody Clone: TG86 + P/T1
Clone TG86 + P/T1 Host and Isotype: Mouse IgM
Anti-Human, Rabbit, and Zebrafish TGF-alpha Positive Control Sample: Jurkat or Ramos cells. Testicular Carcinoma
Cellular Localization of Antibody Cytoplasmic, Extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFalpha- (TG86 & P/T1)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

TGF-alpha Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TGFA antibody staining is expected to be primarily localized to the vesicles. There is variability in either the signal strength or the localization of signal in vesicles from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in TGF-alpha antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of TGF-alpha could be seen inlymphoid tissue in appendix, hematopoietic cells in the bone marrow, glial cells in the caudate nucleus, cells in the granular layer in cerebellum, endothelial cells in the colon, glandular cells in the endometrium, neuronal cells in the hippocampus, pneumocytes in lung, germinal center cells in the lymph node, squamous epithelial cells in the oral mucosa, epidermal cells in the skin, smooth muscle cells in the smooth muscle, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue and non-germinal center cells in the tonsil. We were unable to detect TGF-alpha in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of TGF-alpha expression as measured by anti-TGF-alpha antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + + ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ +
TGFA Variability ++ ++ +++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio’s TGF-alpha Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-TGF-alpha Antibody Clone TG86 + P/T1

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