PDF DatasheetMouse Anti-TIGIT / VSTM3 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-TIGIT / VSTM3 Antibody Clone: 1G9
Clone 1G9 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
TIGIT / VSTM3 Previously Observed Antibody Staining Patterns
| TIGIT / VSTM3 General Information | |
|---|---|
| Alternate Names | |
| TIGIT, VSTM3, Vstm3, ENSMUSG71552 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tigit |
| Entrez Gene ID | 100043314 |
| Ensemble Gene ID | ENSMUSG00000071552 |
| RefSeq Protein Accession(s) | NP_001139797, XP_017172323 |
| RefSeq mRNA Accession(s) | XM_017316834, NM_001146325 |
| RefSeq Genomic Accession(s) | NC_000082 |
| UniProt ID(s) | A0A0B4J1G6 |
| UniGene ID(s) | A0A0B4J1G6 |
| Cosmic ID(s) | Tigit |
| KEGG Gene ID(s) | mmu:100043314 |
| General Description of TIGIT / VSTM3. | |
| The 1G9 antibody reacts with mouse TIGIT (T cell Ig and ITIM domain), a 26 kDa member of the CD28 receptor family which is reported to regulate T cell receptor (TCR) activation. Within the CD28 family of receptors there are those which have co-stimulatory activity, such as CD28 and CTLA-4, as well as more recently identified receptors like TIGIT which are proposed to provide co-inhibitory signals. TIGIT is expressed and upregulated on activated T cells, and is also expressed on memory and regulatory T cells. Upon engagement by its ligands, CD112 and CD155, TIGIT signaling inhibits T cell proliferation and suppresses T cell responses, without triggering cell deletion. A second inhibitory effect of TIGIT signaling is the generation of immunoregulatory dendritic cells, which secrete IL-10 and TGF-beta to further inhibit T cell function.The 1G9 antibody may be used for flow cytometric analysis of TIGIT, which is expressed at very high levels on T regulatory cells (Tregs) and activated conventional T cells, as well as memory T cells and NK cells. | |
Selected References
Joller N, Peters A, Anderson AC, and Kuchroo VK. 2012. Immunol. Rev. 248(1):122-139. (Flow cytometry)Joller N, Hafler JP, Brynedal B, Kassam N, Spoerl S, Levin SD, Sharpe AH, and Kuchroo VK. 2011. J. Immunol. 186: 1338-1342. (Flow cytometry)
Limitations and Warranty
enQuire Bio’s Mouse Anti-TIGIT Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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