PDF DatasheetHuman and Rat Anti-TNF-alpha Antibody Product Attributes
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (2-4ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-TNF-alpha Antibody Clone: TNFA/1172
Clone TNFA/1172 Host and Isotype: Mouse IgM kappa
Anti-Human and Rat TNF-alpha Positive Control Sample: HeLa, HL-60, or A431 cells. Pancreas or Histiocytoma
Cellular Localization of Antibody Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human TNF-alpha protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
TNF-alpha Previously Observed Antibody Staining Patterns
| TNF-alpha General Information | |
|---|---|
| Alternate Names | |
| Tumor necrosis factor, TNF, TNFalpha, TNFa, TNF alpha, TNF? | |
| Molecular Weight | |
| 17kDa | |
| Chromosomal Location | |
| 6p21.33 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TNF |
| Entrez Gene ID | 7124 |
| Ensemble Gene ID | ENSG00000232810, ENSG00000230108, ENSG00000223952, ENSG00000206439, ENSG00000228849, ENSG00000228978, ENSG00000228321, ENSG00000204490 |
| RefSeq Protein Accession(s) | NP_000585 |
| RefSeq mRNA Accession(s) | NM_000594 |
| RefSeq Genomic Accession(s) | NG_167244, NG_167248, NC_018917, NG_167246, NG_167247, NG_007462, NG_113891, NG_167245, NG_167249, NC_000006 |
| UniProt ID(s) | Q5STB3, P01375 |
| UniGene ID(s) | Q5STB3, P01375 |
| HGNC ID(s) | 11892 |
| Cosmic ID(s) | TNF |
| KEGG Gene ID(s) | hsa:7124 |
| PharmGKB ID(s) | PA435 |
| General Description of TNF-alpha. | |
| This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages,, causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts, other cell lines, activating polymorphonuclear neutrophils, osteoclasts,, induction of interleukin 1, prostaglandin E2, collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers, AIDS Related Complex. | |
Limitations and Warranty
enQuire Bio’s TNF-alpha Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.