Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/2452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

HER-2 / c-erbB-2 / neu / CD340 Ultraspecific Antibody

Tested against >20,000 Human Proteins

$ 199.00 – $ 429.00

SKU: 2064-MSM12
Species: Human
Tested Applications: IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a, kappa
Mass Spec Validated?: Not MS Validated

SKU: 2064-MSM12-P0 Categories: , , , , , , , , Tag:
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Human Anti-HER-2 / c-erbB-2 / neu / CD340 Antibody Product Attributes

Species: Human
Tested Applications: IHC.
Clonality: Monoclonal
Anti-HER-2 / c-erbB-2 / neu / CD340 Antibody Clone: ERBB2/2452
Clone ERBB2/2452 Host and Isotype: Mouse IgG2a, kappa
Anti-Human HER-2 / c-erbB-2 / neu / CD340 Positive Control Sample: SKBR-3 Cells or Breast Cancers.
Cellular Localization of Antibody ERBB2/2452 Staining: Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide. Also available without BSA & Azide.
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G purified from Bioreactor concentrate.
Immunogen: Recombinant human HER-2 protein fragment (apprx. aa 311-462) (Please call for additional information.)
Storage Conditions: Store at 2 to 8 C (refrigerate). Stable for 24 months when properly stored.

HER-2 / c-erbB-2 / neu / CD340 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ERBB2 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in HER2 / ErbB2 / neu / CD340 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of HER2 / ErbB2 / neu / CD340 could be seen inglandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine and thyroid gland, hematopoietic cells in the bone marrow, hepatocytes in liver, melanocytes in skin, pneumocytes in lung, squamous epithelial cells in the cervix, uterine, esophagus, tonsil and vagina and glandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle and small intestine and thyroid gland. We were unable to detect HER2 / ErbB2 / neu / CD340 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of HER2 / ErbB2 / neu / CD340 expression as measured by anti-HER2 / ErbB2 / neu / CD340 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ – – – – – – + – – – – – – – – – – – +
ERBB2 Variability +++ + + ++ ++ + ++ ++ + + + ++ ++ + + ++ + ++ + ++

Limitations and Warranty

enQuire Bio’s product, HER-2 / c-erbB-2 / neu / CD340 MonoSpecific Antibody, is available for Research Use Only (RUO-Only). This antibody is guaranteed to work for a period of two years when properly stored.
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