PDF DatasheetHuman CD73 / NT5E Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CD73 / NT5E based upon sequence from Human
Host: QP2192 protein expressed in Human.
Tag: C-terminal His
Protein Construction: A DNA sequence enoding full length human CD73 plus a c-terminal 6x histidine tag was expressed in human HEK293 cells.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP2192.
Bioactivity Data: Measured by its ability to hydrolyze the 5′-phosphate group from the substrate adenosine 5′-monophosphate (AMP). The specific activity is > 20,000 pmoles/min/ug.
Full Length? Full Length
Expression Region: Trp27-Asp547
Amino Acid Sequence: WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHA DETFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KVD
Purity: > 95 % as determined by SDS-PAGE
Reconstitution Instructions: Ships in liquid format.
Concentration of Human CD73 / NT5E Protein:
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: 0.22 um filtered solution of TBS plus 4mM CaCl2 and 20% Glycerol at pH 7.5
Storage Conditions: The protein should be aliquoted and stored under sterile conditions at -20 C short-term and -70 C for periods longer than one month. Avoid repeated freeze-thaw cycles.
| Recombinant Human CD73 Protein (His Tag) General Information | |
|---|---|
| Alternate Names | |
| CALJA, CD73, E5NT, CD 73 , NT5E | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | NT5E |
| Entrez Gene ID | 4907 |
| Ensemble Gene ID | ENSG00000135318 |
| RefSeq Protein Accession(s) | NP_001191742.1 |
| RefSeq mRNA Accession(s) | NM_001204813.1, NM_002526.3 |
| UniProt ID(s) | P21589 |
| UniGene ID(s) | Hs.153952 |
| HGNC ID(s) | HGNC:8021 |
| COSMIC ID Link(s) | NT5E |
| KEGG Gene ID(s) | hsa:4907 |
| PharmGKB ID(s) | PA31804 |
Limitations and Performance Guarantee
This product is for Research Use Only. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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