PDF DatasheetHuman VEGF-165 Isoform Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant VEGF-165 Isoform based upon sequence from: Human
Host: QP5211 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5211.
Bioactivity Data: HUVEC proliferation is significantly induced by less than 10 ng/ml of Human VEGF-165 Isoform, or activity of at least 1.0 x 10^5 units/mg. Typical ED50 values are less than 5 ng/ml.
Monomer or Dimer: Dimer
Amino Acid Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human VEGF-165 Isoform Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: Sterile filtered (0.22 um) solution containing 0.1% TFA
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Human VEGF-165 Isoform Protein (Animal Free) General Information | |
|---|---|
| Alternate Names | |
| VEGF, VEGFA, VEGF165, MVCD1, VPF, VEGF | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | VEGFA |
| Ensemble Gene ID | ENSG00000112715 |
| RefSeq Protein Accession(s) | NP_001165097.1 |
| RefSeq mRNA Accession(s) | NM_001171626.1 |
| UniProt ID(s) | P15692-4 |
| COSMIC ID Link(s) | VEGFA |
| General Description of Recombinant Human VEGF-165 Isoform Protein (Animal Free). | |
| Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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