PDF DatasheetMouse PDGF-B Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PDGF-B based upon sequence from Mouse
Host: QP5465 protein expressed in E. coli.
Tag: Untagged
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP5465.
Bioactivity Data: 3T3 cell proliferation significantly induced by less than 2 ng/ml of Mouse PDGF-B.
Monomer or Dimer: Dimer
Amino Acid Sequence: MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: A Certificate of Analysis (CofA) will be sent along with the protein and will contain the proper reconstitution instructions.
Concentration of Mouse PDGF-B Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than or equal to 1.00 EUs/ug as determined by the LAL method.
Buffer: 10 mM sodium citrate, pH 3.0
Storage Conditions: Store at -20C to -80C lyophilized. Guaranteed for 1 year at -80C reconstituted and 3 months at 4C reconstituted.
| Recombinant Mouse PDGF-B Protein General Information | |
|---|---|
| Alternate Names | |
| PDGF-B, IBGC5, PDGF2, SSV, PDGF-2, SIS, c-sis | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PDGFB |
| Entrez Gene ID | 18591 |
| RefSeq Protein Accession(s) | NP_035187.2 |
| RefSeq mRNA Accession(s) | NM_011057.3, XM_017316502.1 |
| UniProt ID(s) | P31240 |
| UniGene ID(s) | Mm.144089 |
| COSMIC ID Link(s) | PDGFB |
| KEGG Gene ID(s) | mmu:18591 |
| General Description of Recombinant Mouse PDGF-B Protein . | |
| Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by interleukin 1 (IL-1), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF?), transforming growth factor beta (TGF-?), and epidermal growth factor (EGF) signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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