PDF DatasheetHuman E3 ubiquitin-protein ligase RFWD2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant E3 ubiquitin-protein ligase RFWD2 based upon sequence from: Human
Host: QP10010 protein expressed in Invitro-E.coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens(Human) E3 ubiquitin-protein ligase RFWD2, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10010.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Met1 – Val731
Amino Acid Sequence: MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AVSAAALVSG GVAQAAGSGG LGGPVRPVLV APAVSGSGGG AVSTGLSRHS CAARPSAGVG GSSSSLGSGS RKRPLLAPLC NGLINSYEDK SNDFVCPICF DMIEEAYMTK CGHSFCYKCI HQSLEDNNRC PKCNYVVDNI DHLYPNFLVN ELILKQKQRF EEKRFKLDHS VSSTNGHRWQ IFQDWLGTDQ DNLDLANVNL MLELLVQKKK QLEAESHAAQ LQILMEFLKV ARRNKREQLE QIQKELSVLE EDIKRVEEMS GLYSPVSEDS TVPQFEAPSP SHSSIIDSTE YSQPPGFSGS SQTKKQPWYN STLASRRKRL TAHFEDLEQC YFSTRMSRIS DDSRTASQLD EFQECLSKFT RYNSVRPLAT LSYASDLYNG SSIVSSIEFD RDCDYFAIAG VTKKIKVYEY DTVIQDAVDI HYPENEMTCN SKISCISWSS YHKNLLASSD YEGTVILWDG FTGQRSKVYQ EHEKRCWSVD FNLMDPKLLA SGSDDAKVKL WSTNLDNSVA SIEAKANVCC VKFSPSSRYH LAFGCADHCV HYYDLRNTKQ PIMVFKGHRK AVSYAKFVSG EEIVSASTDS QLKLWNVGKP YCLRSFKGHI NEKNFVGLAS NGDYIACGSE NNSLYLYYKG LSKTLLTFKF DTVKSVLDKD RKEDDTNEFV SAVCWRALPD GESNVLIAAN SQGTIKVLEL V
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human E3 ubiquitin-protein ligase RFWD2 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human E3 ubiquitin-protein ligase RFWD2 Protein General Information | |
|---|---|
| Alternate Names | |
| RNF200; COP1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | RFWD2 |
| Entrez Gene ID | 64326 |
| Ensemble Gene ID | ENSG00000143207 |
| RefSeq Protein Accession(s) | NP_001001740.1 |
| RefSeq mRNA Accession(s) | NM_001001740.3, NM_001286644.1, NM_022457.6 |
| UniProt ID(s) | Q8NHY2 |
| UniGene ID(s) | Hs.523744 |
| HGNC ID(s) | HGNC:17440 |
| COSMIC ID Link(s) | RFWD2 |
| KEGG Gene ID(s) | hsa:64326 |
| PharmGKB ID(s) | PA134952161 |
| General Description of Recombinant Human E3 ubiquitin-protein ligase RFWD2 Protein. | |
| E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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