PDF DatasheetHuman JNK1 / MAPK8 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant JNK1 / MAPK8 based upon sequence from: Human
Host: QP10043 protein expressed in Baculovirus.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) JNK1 / MAPK8, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10043.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Ser4 – Pro422a
Amino Acid Sequence: SKRDNNFYSV EIGDSTFTVL KRYQNLKPIG SGAQGIVCAA YDAILERNVA IKKLSRPFQN QTHAKRAYRE LVLMKCVNHK NIIGLLNVFT PQKSLEEFQD VYIVMELMDA NLCQVIQMEL DHERMSYLLY QMLCGIKHLH SAGIIHRDLK PSNIVVKSDC TLKILDFGLA RTAGTSFMMT PYVVTRYYRA PEVILGMGYK ENVDLWSVGC IMGEMVCHKI LFPGRDYIDQ WNKVIEQLGT PCPEFMKKLQ PTVRTYVENR PKYAGYSFEK LFPDVLFPAD SEHNKLKASQ ARDLLSKMLV IDASKRISVD EALQHPYINV WYDPSEAEAP PPKIPDKQLD EREHTIEEWK ELIYKEVMDL EERTKNGVIR GQPSPLGAAV INGSQHPSSS SSVNDVSSMS TDPTLASDTD SSLEAAAGP
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human JNK1 / MAPK8 Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human JNK1 / MAPK8 Protein General Information | |
|---|---|
| Alternate Names | |
| JNK1; MAPK8; JNK21B1/2; JNK-46; SAPK1c; JNK1; JNK; JNK1A2; PRKM8; SAPK1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAPK8 |
| Entrez Gene ID | 5599 |
| Ensemble Gene ID | ENSG00000107643 |
| RefSeq Protein Accession(s) | NP_001265476.1 |
| RefSeq mRNA Accession(s) | NM_001278547.1, NM_001278548.1, NM_001323302.1, NM_001323321.1, NM_001323322.1, NM_001323323.1, NM_001323324.1, NM_001323325.1, NM_001323326.1, NM_001323327.1, NM_001323328.1, NM_001323329.1, NM_001323330.1, NM_001323331.1, NM_139046.3, NM_139049.3 |
| UniProt ID(s) | P45983 |
| UniGene ID(s) | Hs.138211 |
| HGNC ID(s) | HGNC:6881 |
| COSMIC ID Link(s) | MAPK8 |
| KEGG Gene ID(s) | hsa:5599 |
| PharmGKB ID(s) | PA283 |
| General Description of Recombinant Human JNK1 / MAPK8 Protein. | |
| Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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