PDF DatasheetHuman NAD-dependent malic enzyme, mitochondrial Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant NAD-dependent malic enzyme, mitochondrial based upon sequence from: Human
Host: QP10156 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Human NAD-dependent malic enzyme, mitochondrial, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10156.
Bioactivity Data: Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS
PLEKYIYIM GIQERNEKLF YRILQDDIES LMPIVYTPTV GLACSQYGHI FRRPKGLFIS I
SDRGHVRS IVDNWPENHV KAVVVTDGER ILGLGDLGVY GMGIPVGKLC LYTACAGIRP DR
CLPVCID VGTDNIALLK DPFYMGLYQK RDRTQQYDDL IDEFMKAITD RYGRNTLIQF EDF
GNHNAF RFLRKYREKY CTFNDDIQGT AAVALAGLLA AQKVISKPIS EHKILFLGAG EAAL
GIANL IVMSMVENGL SEQEAQKKIW MFDKYGLLVK GRKAKIDSYQ EPFTHSAPES IPDTF
EDAV NILKPSTIIG VAGAGRLFTP DVIRAMASIN ERPVIFALSN PTAQAECTAE EAYTLT
EGR CLFASGSPFG PVKLTDGRVF TPGQGNNVYI FPGVALAVIL CNTRHISDSV FLEAAKA
LT SQLTDEELAQ GRLYPPLANI QEVSINIAIK VTEYLYANKM AFRYPEPEDK AKYVKERT
W RSEYDSLLPD VYEWPESASS PPVITEHHHH HH>
PLEKYIYIM GIQERNEKLF YRILQDDIES LMPIVYTPTV GLACSQYGHI FRRPKGLFIS I
SDRGHVRS IVDNWPENHV KAVVVTDGER ILGLGDLGVY GMGIPVGKLC LYTACAGIRP DR
CLPVCID VGTDNIALLK DPFYMGLYQK RDRTQQYDDL IDEFMKAITD RYGRNTLIQF EDF
GNHNAF RFLRKYREKY CTFNDDIQGT AAVALAGLLA AQKVISKPIS EHKILFLGAG EAAL
GIANL IVMSMVENGL SEQEAQKKIW MFDKYGLLVK GRKAKIDSYQ EPFTHSAPES IPDTF
EDAV NILKPSTIIG VAGAGRLFTP DVIRAMASIN ERPVIFALSN PTAQAECTAE EAYTLT
EGR CLFASGSPFG PVKLTDGRVF TPGQGNNVYI FPGVALAVIL CNTRHISDSV FLEAAKA
LT SQLTDEELAQ GRLYPPLANI QEVSINIAIK VTEYLYANKM AFRYPEPEDK AKYVKERT
W RSEYDSLLPD VYEWPESASS PPVITEHHHH HH>
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human NAD-dependent malic enzyme, mitochondrial Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2um filtered PBS, pH 7.4. , lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human NAD-dependent malic enzyme, mitochondrial Protein General Information | |
|---|---|
| Alternate Names | |
| ODS1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ME2 |
| Entrez Gene ID | 4200 |
| Ensemble Gene ID | ENSG00000082212 |
| RefSeq Protein Accession(s) | NP_001161807.1 |
| RefSeq mRNA Accession(s) | NM_001168335.1, NM_002396.4 |
| UniProt ID(s) | P23368 |
| UniGene ID(s) | Hs.233119 |
| HGNC ID(s) | HGNC:6984 |
| COSMIC ID Link(s) | ME2 |
| KEGG Gene ID(s) | hsa:4200 |
| PharmGKB ID(s) | PA30724 |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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