PDF DatasheetHuman Fructose-2,6-bisphosphatase TIGAR Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Fructose-2,6-bisphosphatase TIGAR based upon sequence from: Human
Host: QP10157 protein expressed in E. coli.
Tag: TAT
Protein Construction: A DNA sequence encoding the Human Fructose-2,6-bisphosphatase TIGAR, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10157.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 ug/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK
FTHAFSSDL MRTKQTMHGI LERSKFCKDM TVKYDSRLRE RKYGVVEGKA L
SELRAMAK AAREECPVFT PPGGETLDQV KMRGIDFFEF LCQLILKEAD QK
EQFSQGS PSNCLETSLA EIFPLGKNHS SKVNSDSGIP GLAASVLVVS HGA
YMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKP
TVQCI CMNLQDHLNG LTETRGGYGR KKRRQ
FTHAFSSDL MRTKQTMHGI LERSKFCKDM TVKYDSRLRE RKYGVVEGKA L
SELRAMAK AAREECPVFT PPGGETLDQV KMRGIDFFEF LCQLILKEAD QK
EQFSQGS PSNCLETSLA EIFPLGKNHS SKVNSDSGIP GLAASVLVVS HGA
YMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKP
TVQCI CMNLQDHLNG LTETRGGYGR KKRRQ
Purity: Greater than 96% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human Fructose-2,6-bisphosphatase TIGAR Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 m filtered 30 % Acetonitrile, 0.1% TFA, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Fructose-2,6-bisphosphatase TIGAR Protein General Information | |
|---|---|
| Alternate Names | |
| C12orf5; FR2BP | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TIGAR |
| Entrez Gene ID | 57103 |
| Ensemble Gene ID | ENSG00000078237 |
| RefSeq Protein Accession(s) | NP_065108.1 |
| RefSeq mRNA Accession(s) | NM_020375.2 |
| UniProt ID(s) | Q9NQ88 |
| UniGene ID(s) | Hs.504545 |
| HGNC ID(s) | HGNC:1185 |
| KEGG Gene ID(s) | hsa:57103 |
| PharmGKB ID(s) | PA25506 |
| General Description of Recombinant Human Fructose-2,6-bisphosphatase TIGAR Protein. | |
| Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (By similarity). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (By similarity). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973). | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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