PDF DatasheetHuman LCN2 / NGAL Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LCN2 / NGAL based upon sequence from: Human
Host: QP10190 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human LCN2 / NGAL, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10190.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human LCN2 / NGAL Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered 20 mM PBS, pH 7.4, 150mM NaCl, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human LCN2 / NGAL Protein General Information | |
|---|---|
| Alternate Names | |
| LCN2; NGAL; AW212229; Sip24; 24p3; NRL | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | LCN2 |
| Entrez Gene ID | 3934 |
| Ensemble Gene ID | ENSG00000148346 |
| RefSeq Protein Accession(s) | NP_005555.2 |
| RefSeq mRNA Accession(s) | NM_005564.4 |
| UniProt ID(s) | P80188 |
| UniGene ID(s) | Hs.204238 |
| HGNC ID(s) | HGNC:6526 |
| COSMIC ID Link(s) | LCN2 |
| KEGG Gene ID(s) | hsa:3934 |
| PharmGKB ID(s) | PA30309 |
| General Description of Recombinant Human LCN2 / NGAL Protein. | |
| Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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