PDF DatasheetHuman IGFBP3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IGFBP3 based upon sequence from: Human
Host: QP10386 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human IGFBP3, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10386.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 103 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
Purity: Greater than 98% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human IGFBP3 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human IGFBP3 Protein General Information | |
|---|---|
| Alternate Names | |
| IGFBP3; BP-53; IBP3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGFBP3 |
| Entrez Gene ID | 3486 |
| Ensemble Gene ID | ENSG00000146674 |
| RefSeq Protein Accession(s) | NP_000589.2 |
| RefSeq mRNA Accession(s) | NM_000598.4, NM_001013398.1, XM_017012152.1 |
| UniProt ID(s) | P17936 |
| HGNC ID(s) | HGNC:5472 |
| COSMIC ID Link(s) | IGFBP3 |
| KEGG Gene ID(s) | hsa:3486 |
| PharmGKB ID(s) | PA29705 |
| General Description of Recombinant Human IGFBP3 Protein. | |
| IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.