PDF DatasheetHuman VEGF / VEGFA / VEGF165 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant VEGF / VEGFA / VEGF165 based upon sequence from: Human
Host: QP10395 protein expressed in E. coli.
Tag: Untagged
Protein Construction: A DNA sequence encoding the Human VEGF / VEGFA / VEGF165, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP10395.
Bioactivity Data: Equivalent or superior bioactivity to competitors. Data available upon request. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.
Full Length? Partial (see sequence information for more details).
Amino Acid Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Purity: Greater than 95% as determined by SDS-PAGE and HPLC.
Reconstitution Instructions:
Concentration of Human VEGF / VEGFA / VEGF165 Protein: Lyophilized. Concentration determined by reconstitution volume.
Endotoxin Levels: Less than 1.0 EU/ug as determined by LAL method.
Buffer: 0.2 um filtered PBS, pH 7.4, lyophilized
Storage Conditions: Store at -20C to -80C.
| Recombinant Human VEGF / VEGFA / VEGF165 Protein General Information | |
|---|---|
| Alternate Names | |
| VEGF; VEGFA; VEGF165; wu:fj82c06; vegf; vegfa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | VEGFA |
| Entrez Gene ID | 7422 |
| Ensemble Gene ID | ENSG00000112715 |
| RefSeq Protein Accession(s) | NP_001020537.2 |
| RefSeq mRNA Accession(s) | NM_001025366.2, NM_001025367.2, NM_001025368.2, NM_001025369.2, NM_001025370.2, NM_001033756.2, NM_001171622.1, NM_001171623.1, NM_001171624.1, NM_001171625.1, NM_001171626.1, NM_001171627.1, NM_001171628.1, NM_001171629.1, NM_001171630.1, NM_001204384.1, NM_001204385.1, NM_001287044.1, NM_001317010.1, NM_003376.5 |
| UniProt ID(s) | P15692 |
| HGNC ID(s) | HGNC:12680 |
| COSMIC ID Link(s) | VEGFA |
| KEGG Gene ID(s) | hsa:7422 |
| PharmGKB ID(s) | PA37302 |
| General Description of Recombinant Human VEGF / VEGFA / VEGF165 Protein. | |
| Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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