PDF DatasheetHuman ACP5 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant ACP5 based upon sequence from Human
Host: QP10502 protein expressed in Insect.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of ACP5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10502.
Bioactivity Data: Specific activity is >10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37°C.
Amino Acid Sequence: ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: ACP5 protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human ACP5 General Information | |
|---|---|
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| Alternate Names | |
| Acid Phosphatase 5, Tartrate Resistant, Tartrate-Resistant Acid ATPase, Human Purple Acid Phosphatase, EC 3.1. 3.2, TrATPase, Tartrate-Resistant Acid Phosphatase Type 5, Tartrate-Resistant Acid Phosphatase 5a, Tartrate-Resistant Acid Phosphatase 5b, Tartrate-Resistant Acid Phosphatase, Type 5 Acid Phosphatase, TRACP5a, TRACP5b, TR-AP, HPAP, TRAP, ACP5. | |
| Molecular Weight | |
| 34.3 kDa | |
| Chromosomal Location | |
| p13.2 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ACP5 |
| Entrez Gene ID | 54 |
| UniProt ID(s) | P13686 |
| COSMIC ID Link(s) | ACP5 |
| KEGG Gene ID(s) | hsa:54 |
| General Description of Recombinant Human ACP5. | |
| Human ACP5 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 304 amino acids (22-325 AA ) and having a molecular mass of 34.3kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions). ACP5 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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