PDF DatasheetHuman ADA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant ADA based upon sequence from Human
Host: QP10505 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ADA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10505.
Bioactivity Data: Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: ADA protein 0.5 mg/ml is supplied in 20mM Tris-HCL, pH 8.0, 1mM DTT and 20% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: ADA is supplied as a sterile filtered clear solution.
| Recombinant Human ADA General Information | |
|---|---|
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| Alternate Names | |
| Adenosine deaminase, Adenosine aminohydrolase, ADA1, EC 3.5. 4.4. | |
| Molecular Weight | |
| 42.9 kDa | |
| Chromosomal Location | |
| q13.12 on chromosome 20 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ADA |
| Entrez Gene ID | 100 |
| UniProt ID(s) | P00813 |
| COSMIC ID Link(s) | ADA |
| KEGG Gene ID(s) | hsa:100 |
| General Description of Recombinant Human ADA. | |
| Human ADA produced in E. coli is a single, non-glycosylated polypeptide chain containing 383 amino acids (1-363) and having a molecular mass of 42.9 kDa. The ADA is fused to a 20 amino acid His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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