PDF DatasheetHuman ADK Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant ADK based upon sequence from Human
Host: QP10507 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ADK was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10507.
Bioactivity Data: Specific activity is > 30 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKARVCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPDFH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The ADK protein solution (0.5 mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 1mM EDTA & 50mM NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human ADK General Information | |
|---|---|
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| Alternate Names | |
| Adenosine 5′-phosphotransferase, EC 2.7. 1.20, AK, ADK, Adenosine Kinase, Adenosine 5-Phosphotransferase, Testicular Tissue Protein Li 14, EC 2.7. 1. | |
| Molecular Weight | |
| 40.5 kDa | |
| Chromosomal Location | |
| q22.2 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ADK |
| Entrez Gene ID | 132 |
| UniProt ID(s) | P55263 |
| COSMIC ID Link(s) | ADK |
| KEGG Gene ID(s) | hsa:132 |
| General Description of Recombinant Human ADK. | |
| ADK produced in E. Coli is a single, non-glycosylated polypeptide chain containing 362 amino acids (22-362AA ) and having a molecular mass of 40.5kDa. ADK is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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