PDF DatasheetHuman ALDH2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant ALDH2 based upon sequence from Human
Host: QP10515 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of ALDH2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10515.
Bioactivity Data: Specific activity was found to be > 250pmol/min/ug, and was obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25°C.
Amino Acid Sequence: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLNRLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAGTVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: ALDH2 protein (1 mg/ml) contains 20mM Tris-HCl buffer, pH 7.5, 1mM DTT, 1mM EDTA and 10% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human ALDH2 General Information | |
|---|---|
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| Alternate Names | |
| ALDM, ALDHI, ALDH-E2, MGC1806, ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH class 2. | |
| Molecular Weight | |
| 54.5 kDa | |
| Chromosomal Location | |
| q24.12 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ALDH2 |
| Entrez Gene ID | 217 |
| UniProt ID(s) | P05091 |
| COSMIC ID Link(s) | ALDH2 |
| KEGG Gene ID(s) | hsa:217 |
| General Description of Recombinant Human ALDH2. | |
| Human ALDH2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 501 amino acids (18-517 AA ) & having a molecular mass of 54.5 kDa. The ALDH2 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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