PDF DatasheetMouse BD 3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BD 3 based upon sequence from Mouse
Host: QP10532 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BD 3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Beta Defensin-3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20µg/ml, corresponding to a specific activity of 50,000-250,000units/mg.
Amino Acid Sequence: KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Mouse BD-3 was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized Beta Defensin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse BD 3 General Information | |
|---|---|
| Alternate Names | |
| Beta-defensin 3, BD-3, mBD-3, Defensin beta 3, Defb3, Bd3, MGC129397. | |
| Molecular Weight | |
| 4.6 kDa | |
| Chromosomal Location | |
| A1.3 on chromosome 8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Defb3 |
| Entrez Gene ID | 27358 |
| UniProt ID(s) | Q9WTL0 |
| COSMIC ID Link(s) | Defb3 |
| KEGG Gene ID(s) | mmu:27358 |
| General Description of Recombinant Mouse BD 3. | |
| Mouse Beta Defensin-3 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.6kDa. The BD-3 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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