PDF DatasheetRat BD 3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BD 3 based upon sequence from Rat
Host: QP10533 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BD 3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized BD-3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20µg/ml, corresponding to a specific activity of 50,000-250,000units/mg.
Amino Acid Sequence: KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: BD-3 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat BD 3 General Information | |
|---|---|
| Alternate Names | |
| Beta-defensin 3, BD-3, Defensin beta 3, Defb3. | |
| Molecular Weight | |
| 4.5 kDa | |
| Chromosomal Location | |
| q12.5 on chromosome 16 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Defb3 |
| Entrez Gene ID | 641623 |
| UniProt ID(s) | Q32ZI4 |
| COSMIC ID Link(s) | Defb3 |
| KEGG Gene ID(s) | rno:641623 |
| General Description of Recombinant Rat BD 3. | |
| Rat BD-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5kDa. The BD-3 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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