PDF DatasheetHuman CA13 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CA13 based upon sequence from Human
Host: QP10542 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CA13 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10542.
Bioactivity Data: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSP MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: CA13 protein solution (0.5 mg/ml) containing Phosphate buffered saline (pH 7.4),10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human CA13 General Information | |
|---|---|
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| Alternate Names | |
| Carbonic Anhydrase XIII, Carbonate Dehydratase XIII, EC 4.2. 1.1, CA-XIII, Carbonic, Anhydrase 13, CAXIII. | |
| Molecular Weight | |
| 31.8 kDa | |
| Chromosomal Location | |
| q21.2 on chromosome 8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CA13 |
| Entrez Gene ID | 377677 |
| UniProt ID(s) | Q8N1Q1 |
| COSMIC ID Link(s) | CA13 |
| KEGG Gene ID(s) | hsa:377677 |
| General Description of Recombinant Human CA13. | |
| Human CA13 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-262AA) and having a molecular mass of 31.8kDa. CA13 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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