PDF DatasheetHuman CDA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CDA based upon sequence from Human
Host: QP10546 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CDA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10546.
Bioactivity Data: Specific activity :> 3.5unit/mg. One unit of activity was defined as the amount required to deaminate 1.0 umole of cytidine per min at pH 7.5 at 250C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The CDA solution (0.5 mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0), 1mM DTT, 2mM EDTA, 100mM NaCl and 40% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human CDA General Information | |
|---|---|
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|
| Alternate Names | |
| Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD. | |
| Molecular Weight | |
| 18.3 kDa | |
| Chromosomal Location | |
| p36.12 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDA |
| Entrez Gene ID | 978 |
| UniProt ID(s) | P32320 |
| COSMIC ID Link(s) | CDA |
| KEGG Gene ID(s) | hsa:978 |
| General Description of Recombinant Human CDA. | |
| Human CDA fused with a 20 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 AA ) and having a molecular mass of 18.3kDa. The CDA is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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