PDF DatasheetHuman CKB Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CKB based upon sequence from Human
Host: QP10552 protein expressed in Pichia Pastoris.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of CKB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10552.
Bioactivity Data: The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No. 45-UV, 1IU-1 µmole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.
Monomer or Dimer: Dimer
Amino Acid Sequence: MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: CKBB Human contains 10Mm Bis-Tris-HCl pH 6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.
Storage Conditions: CKBB should be stored below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colourless liquid formulation.
| Recombinant Human CKB General Information | |
|---|---|
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| Alternate Names | |
| Creatine kinase B-type, EC 2.7. 3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI. | |
| Molecular Weight | |
| 42.6 kDa | |
| Chromosomal Location | |
| q32.33 on chromosome 14 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CKB |
| Entrez Gene ID | 1152 |
| UniProt ID(s) | P12277 |
| COSMIC ID Link(s) | CKB |
| KEGG Gene ID(s) | hsa:1152 |
| General Description of Recombinant Human CKB. | |
| Human CKBB produced in Pichia Pastoris is a dimeric glycosylated full length polypeptide chain comprised of 2 identical B subunits and having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and having a MW of 47kDa The CKBB is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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