PDF DatasheetHuman CKMT1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CKMT1 based upon sequence from Human
Host: QP10555 protein expressed in Pichia Pastoris.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of CKMT1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10555.
Bioactivity Data: The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No. 45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.
Amino Acid Sequence: MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K
Purity: Greater than 95.0% as determined bya) Analysis by RP-HPLCb) Analysis by SDS-PAGE
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.
Storage Conditions: CKMT1 although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colourless liquid formulation.
| Recombinant Human CKMT1 General Information | |
|---|---|
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| Alternate Names | |
| Creatine kinase M-type, EC 2.7. 3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI. | |
| Molecular Weight | |
| 43.1 kDa | |
| Chromosomal Location | |
| q13.32 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CKM |
| Entrez Gene ID | 1158 |
| UniProt ID(s) | P06732 |
| COSMIC ID Link(s) | CKM |
| KEGG Gene ID(s) | hsa:1158 |
| General Description of Recombinant Human CKMT1. | |
| Human CKMT1 without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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