PDF DatasheetHuman CSTA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CSTA based upon sequence from Human
Host: QP10563 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CSTA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10563.
Bioactivity Data: The IC50 value is < 1.0nM. The inhibitory function of CSTA on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Cystatin-A (1 mg/ml) in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human CSTA General Information | |
|---|---|
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| Alternate Names | |
| Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA. | |
| Molecular Weight | |
| 13.1 kDa | |
| Chromosomal Location | |
| q21.1 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CSTA |
| Entrez Gene ID | 1475 |
| UniProt ID(s) | P01040 |
| COSMIC ID Link(s) | CSTA |
| KEGG Gene ID(s) | hsa:1475 |
| General Description of Recombinant Human CSTA. | |
| Human Cystatin A produced in E. Coli is a single, non-glycosylated, Polypeptide chain containing 118 amino acids (1-98AA ) and having a molecular mass of 13.1 kDa. The Cystatin A is fused to a 20 amino acid His tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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