PDF DatasheetMouse CTSB Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant CTSB based upon sequence from Mouse
Host: QP10566 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CTSB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10566.
Bioactivity Data: Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.
Amino Acid Sequence: HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: CTSB protein solution (0.5 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Mouse CTSB General Information | |
|---|---|
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| Alternate Names | |
| Cathepsin B, Cathepsin B1, CTSB. | |
| Molecular Weight | |
| 36.4 kDa | |
| Chromosomal Location | |
| D1 on chromosome 14 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ctsb |
| Entrez Gene ID | 13030 |
| UniProt ID(s) | P10605 |
| COSMIC ID Link(s) | Ctsb |
| KEGG Gene ID(s) | mmu:13030 |
| General Description of Recombinant Mouse CTSB. | |
| Mouse CTSB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 330 amino acids (18-339AA ) and having a molecular mass of 36.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). CTSB is expressed with an 8 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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