PDF DatasheetMouse Cyclophilin B Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Cyclophilin B based upon sequence from Mouse
Host: QP10569 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Cyclophilin B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10569.
Bioactivity Data: Specific activity is > 1,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris–HCl pH 8.0 using chymotrypsin.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNDKKKG PKVTVKVYFD LQIGDESVGR VVFGLFGKTV PKTVDNFVAL ATGEKGFGYK NSKFHRVIKD FMIQGGDFTR GDGTGGKSIY GERFPDENFK LKHYGPGWVS MANAGKDTNG SQFFITTVKT SWLDGKHVVF GKVLEGMDVV RKVESTKTDSRDKPLKDVII VDSGKIEVEK PFAIAKE
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Cyclophilin B protein solution (1 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Mouse Cyclophilin B General Information | |
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| Alternate Names | |
| Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP. | |
| Molecular Weight | |
| 22.7 kDa | |
| Chromosomal Location | |
| C on chromosome 9 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ppib |
| Entrez Gene ID | 19035 |
| UniProt ID(s) | P24369 |
| COSMIC ID Link(s) | Ppib |
| KEGG Gene ID(s) | mmu:19035 |
| General Description of Recombinant Mouse Cyclophilin B. | |
| Mouse Cyclophilin B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (34-216 AA) and having a molecular mass of 22.7kDa. Cyclophilin B is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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