PDF DatasheetRat Cyclophilin F Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Cyclophilin F based upon sequence from Rat
Host: QP10573 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Cyclophilin F was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10573.
Bioactivity Data: Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in TrisHCl pH 8.0 using chymotrypsin.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Cyclophilin F protein solution (1 mg/ml) containing Phosphate Buffered Saline (pH 7.4), 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Rat Cyclophilin F General Information | |
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| Alternate Names | |
| Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F. | |
| Molecular Weight | |
| 21.8 kDa | |
| Chromosomal Location | |
| p16 on chromosome 16 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ppif |
| Entrez Gene ID | 282819 |
| UniProt ID(s) | P29117 |
| COSMIC ID Link(s) | Ppif |
| KEGG Gene ID(s) | rno:282819 |
| General Description of Recombinant Rat Cyclophilin F. | |
| Rat Cyclophilin F produced in E. Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids (30-206 AA) and having a molecular mass of 21.2Da. Cyclophilin F is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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