PDF DatasheetHuman EPOR Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant EPOR based upon sequence from Human
Host: QP10591 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of EPOR was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10591.
Bioactivity Data: Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect less or equal to 70ng/ml.
Amino Acid Sequence: APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH
Purity: Greater than 95.0% as determined by analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: EPOR protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human EPOR General Information | |
|---|---|
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| Alternate Names | |
| EPO-R, EPOR, Erythropoietin Receptor. | |
| Molecular Weight | |
| 25.6 kDa | |
| Chromosomal Location | |
| p13.2 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EPOR |
| Entrez Gene ID | 2057 |
| UniProt ID(s) | P19235 |
| COSMIC ID Link(s) | EPOR |
| KEGG Gene ID(s) | hsa:2057 |
| General Description of Recombinant Human EPOR. | |
| EPOR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 232 amino acids (25-250AA ) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). EPOR is expressed with a 6 amino acid His-tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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