PDF DatasheetE. Coli G6PD Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant G6PD based upon sequence from E. Coli
Host: QP10622 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of G6PD was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10622.
Bioactivity Data: Specific activity > 70 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction beta- NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Amino Acid Sequence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVDSITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The G6PD protein contains 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant E. Coli G6PD General Information | |
|---|---|
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| Alternate Names | |
| G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase. | |
| Molecular Weight | |
| 55.7 kDa | |
| Chromosomal Location | |
| q28 on chromosome X | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | G6PD |
| Entrez Gene ID | 2539 |
| UniProt ID(s) | P11413 |
| COSMIC ID Link(s) | G6PD |
| KEGG Gene ID(s) | hsa:2539 |
| General Description of Recombinant E. Coli G6PD. | |
| G6PD E. Coli Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 491 amino acids and having a molecular mass of 55.7kDa. The G6PD is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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