PDF DatasheetE. Coli GLDA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GLDA based upon sequence from E. Coli
Host: QP10644 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GLDA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10644.
Bioactivity Data: Specific activity: > 14 Units/ml. One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: GLDA protein solution (1 mg/ml) containing Phosphate buffered saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant E. Coli GLDA General Information | |
|---|---|
| Alternate Names | |
| ECK3937, JW5556, Glycerol dehydrogenase, GDH, GLDH, b3945. | |
| Molecular Weight | |
| 41.1 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | gldA |
| Entrez Gene ID | 948440 |
| UniProt ID(s) | P0A9S5 |
| COSMIC ID Link(s) | gldA |
| KEGG Gene ID(s) | ecj:JW5556; eco:b3945 |
| General Description of Recombinant E. Coli GLDA. | |
| GLDA E. coli Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 390 amino acids (1-367 AA) and having a molecular mass of 41.1kDa. GLDA is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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