PDF DatasheetCanine GM CSF Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GM CSF based upon sequence from Canine
Host: QP10648 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GM CSF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized GMCSF in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF1 erythroleukemic cells is typically 1-4 ng/ml.
Amino Acid Sequence: APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPEGPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQNINFKSFKENL KDFLFNIPFD CWKPVKK
Purity: Greater than 96.0% as determined by1. Analysis by RP-HPLC. 2. Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: GMCSF was lyophilized after extensive dialysis against 1xPBS pH 7.4.
Storage Conditions: Lyophilized GMCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Canine GM CSF General Information | |
|---|---|
| Alternate Names | |
| CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897. | |
| Molecular Weight | |
| 14.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P48749 |
| General Description of Recombinant Canine GM CSF. | |
| GMCSF k9 Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14.2 kDa. GM-CSF is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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