PDF DatasheetHuman GNMT Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant GNMT based upon sequence from Human
Host: QP10649 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GNMT was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10649.
Bioactivity Data: Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: GNMT protein solution (1 mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human GNMT General Information | |
|---|---|
| Alternate Names | |
| Glycine N-methyltransferase, GNMT, Glycine N-Methyltransferase, EC 2.1. 1.20Epididymis Secretory Sperm Binding Protein Li 182mP, HEL-S-182mP. | |
| Molecular Weight | |
| 34.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q14749 |
| General Description of Recombinant Human GNMT. | |
| Human GNMT produced in E. Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295 AA) and having a molecular mass of 34.9kDa. GNMT is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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